ISO 19820:2016 水質—海洋ワムシBrachionusplicatilisに対する急性毒性の測定 | ページ 2

Foreword

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The committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods.

Introduction

The evaluation of harmful effects on chemicals and pollutants on the biota in marine and estuarine environments has, for several years, involved the performance of biological tests.

Rotifers, and especially the species Brachionus plicatilis, are of interest from the ecotoxicological view because they are often an important component of the zooplankton and serve as prey for small fish and larger invertebrates.

The test specified in this International Standard involves determination of the lethal effects of toxicants to the marine rotifer, Brachionus plicatilis, after 24 h or 48 h exposure (depending on the intended use of the results). Prolongation of the exposure to 48 h is advised since it substantially increases the sensitivity of the assay.

1 Scope

This International Standard specifies a method for the determination of the lethal effects of toxicants to Brachionus plicatilis after 24 h or 48 h exposure.

The method is applicable to the following:

• a) chemical substances which are soluble or which can be maintained as a stable suspension of dispersions under the conditions of the test;
• b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration, or centrifugation;
• c) marine or estuarine waters;
• d) sediment elutriates/eluates.

2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

3 Terms and definitions

For the purposes of this document, the following terms and definitions apply.

Bibliography