ISO/TS 21569-9:2025 分子バイオマーカー分析のための水平的手法 — 遺伝子組み換え生物および派生産物の検出のための分析法 — Part 9:P35S-nptII DNA 配列検出のためのコンストラクト特異的リアルタイム PCR ベースのスクリーニング法 | ページ 2

Foreword

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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 16, Horizontal methods for molecular biomarker analysis.

A list of all parts in the ISO 21569 series can be found on the ISO website.

1 Scope

This document specifies a procedure for the detection of the DNA transition sequence between the 35S promoter region from cauliflower mosaic virus (P35S) and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli. The P35S-nptII segment is part of a construct which confers resistance to neomycin/kanamycin antibiotics frequently found in genetically modified (GM) plants.

The detection method is based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific GM plant (event) a follow-up analysis has to be carried out.

This method is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

2 Normative references

The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

3 Terms and definitions

For the purposes of this document, the terms and definitions given in ISO 16577 apply.

ISO and IEC maintain terminology databases for use in standardization at the following addresses:

Bibliography