ISO 13969:2003 ミルクおよび乳製品—微生物阻害剤試験の標準化された説明のためのガイドライン | ページ 2

Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, 2.

The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 13969 | IDF 183 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.

Foreword

IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National Committee in every member country. Every National Committee has the right to be represented on the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO and AOAC International in the development of standard methods of analysis and sampling for milk and milk products.

Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National Committees for voting. Publication as an International Standard requires approval by at least 50 % of IDF National Committees casting a vote.

Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. IDF shall not be held responsible for identifying any or all such patent rights.

ISO 13969 | IDF 183 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.

All work was carried out by the Joint ISO/IDF/AOAC Action Team Antimicrobials and other veterinary medical residues, of the Standing Committee on Analytical methods for additives and contaminants, under the aegis of its project leader, Mrs G. Suhren (DE).

Introduction

The parameters outlined in this International Standard may not need to be evaluated completely for every test, depending on

• a) the field of application of the test under study (e.g. screening or reference method, type of milk, i.e. animal species or raw/heat treated milk),
• b) the information needed [e.g. the introduction of a new substance with fixed maximum residue limit (MRL)], and
• c) the detection pattern (e.g. sensitivity of the test microorganism to a broad or narrow variety of antimicrobial compounds).

Thus “the terms of reference” between the producer and user of a certain test should be agreed upon in the context of these guidelines omitting, for example, those aspects that are not relevant to the intended field of application.

A general disadvantage related to the interpretation of microbial inhibitor tests is that they are usually evaluated in a subjective way and in very few steps, i.e. negative, questionable, and positive by comparison with positive and/or negative control samples.

In cases where the medium contains an indicator, the type of the resultant colour change can depend upon the type of antimicrobial present. This sometimes makes it difficult to obtain a clear distinction between positive and negative results. Test interpretation in few steps also means that small alterations or minor colour developments, which may be of importance in a validation programme, need major experimental effort.

1 Scope

This International Standard gives guidance for a standardized description of microbial inhibitor tests for milk and milk products. It is intended to give a framework and basis for the evaluation/validation of microbial inhibitor tests, allowing the comparison of data obtained from different tests and experimental studies.

2 Terms and definitions

For the purposes of this document, the following terms and definitions apply.