ISO 4833-1:2013 食物連鎖の微生物学—微生物を数えるための水平法—パート1：ポアプレート法による30°Cでのコロニー数 | ページ 2

Foreword

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The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, 2, www.iso.org/directives .

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The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 9, Microbiology.

This first edition, together with ISO 4833-2, cancels and replaces ISO 4833:2003.

ISO 4833 consists of the following parts, under the general title Microbiology of the food chain — Horizontal method for the enumeration of microorganisms:

• Part 1: Colony count at 30 °C by the pour plate technique
• Part 2: Colony count at 30 °C by the surface plating technique

1 Scope

This part of ISO 4833 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to:

• a) products intended for human consumption and for animal feed;
• b) environmental samples in the area of food and feed production and handling.

This part of ISO 4833 is applicable to:

• 1) products that require a reliable count when a low limit of detection is specified (below 10²/g or 10²/ml for liquid samples or below 10³/g for solid samples);
• 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.

The applicability of this part of ISO 4833 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.

For some matrices, the method specified in this part of ISO 4833 can give different results to those obtained using the method specified in ISO 4833-2.

2 Normative references

The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

3 Terms and definitions

For the purposes of this document, the following terms and definitions apply.

Bibliography