ISO 6887-1:2017 食物連鎖の微生物学—微生物学的検査のための試験サンプル、初期懸濁液および小数希釈の準備—パート1：初期懸濁液および小数希釈の準備のための一般規則 | ページ 6

3.1

laboratory sample

sample prepared for sending to the laboratory and intended for inspection or testing

[SOURCE: ISO 7002:1986, A.19]

3.2

composite sample

mixed sample of a number of the same items of food, animal feed, animals or environment, from which a test portion is taken for examination in the laboratory

Note 1 to entry: See illustration of a composite sample in Annex A.

3.3

pooled sample

mixed sample of a number of the same items of food, animal feed, animals or environment, where the complete mixture is the test portion and is taken as a whole for examination in the laboratory

Note 1 to entry: See illustration of a pooled sample in Annex A.

3.4

test sample

sample prepared from the laboratory sample (3.1) according to the procedure specified in the method of test and from which test portions (3.5) are taken

Note 1 to entry: Preparation of the laboratory sample before the test portion is taken is infrequently used in microbiological examinations.

[SOURCE: ISO 7002:1986, A.47]

3.5

test portion

measured (volume or mass) representative sample taken from the laboratory sample (3.1) for use in the preparation of the initial suspension (3.6)

Note 1 to entry: Sometimes preparation of the laboratory sample (3.1) is required before the test portion is taken, but this is infrequently used in microbiological examinations.

3.6

initial suspension

primary dilution

suspension, solution or emulsion obtained after a weighed or measured quantity of the product under examination (or of a test sample prepared from the product) has been mixed with, normally, a nine-fold quantity of diluent, allowing large particles, if present, to settle

Note 1 to entry: Nine-fold quantities of diluent are normally used to produce a decimal dilution series, but other ratios may be required for specific purposes, such as to enumerate low numbers.

3.7

further dilution

suspension or solution obtained by mixing a measured volume of the initial suspension (3.6) with an x-fold volume of diluent and by repeating this operation with further dilutions until a dilution series, suitable for the inoculation of culture media, is obtained

Note 1 to entry: Ten-fold dilutions are normally used to produce a decimal dilution series, but other ratios may be required for specific purposes.

3.8

pooled test portions

mixture of test portions from a number of the same items of food, animal feed, animals or environment, where the complete mixture is the test portion examined

Note 1 to entry: See illustration of pooled test portions in Annex A.

3.9

pooled (pre-)enriched test portions

individually (pre-)enriched test portions from a number of the same items of food, animal feed, animals or environment, from which specified volumes are combined for further examination

Note 1 to entry: See illustration of pooled (pre-)enriched test portions in Annex A.

3.10

specific standard

International Standard or guidance document describing the examination of a specific product (or group of products) for the detection or enumeration of a specific microorganism (or group of microorganisms)